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基于微卫星标记的三疣梭子蟹家系系谱认证 总被引：1，自引：0，他引：1
用6个微卫星标记对三疣梭子蟹(Portunus trituberculatus)的6个家系进行系谱鉴别和遗传多样性研究。6个微卫星标记在6个家系中显示了高度的遗传差异,6个位点的多态信息含量(PIC)分别为Pot09位点0.6045,Pot14位点0.6072,Pot17位点0.8130,Pot18位点0.6870,Pot25位点0.7839,Pot42位点0.6330。6个多态性位点共发现了32个等位基因,每个位点的等位基因数在4～8个之间。不同位点共发现7个家系特异性等位基因,2#和3#家系中各发现2个,1#、5#、6#家系中各发现1个,4#家系未发现特异性等位基因。根据已知亲本及子代基因型,可推断出6个家系中全部亲本的基因型,据此鉴别各家系。在Pot18位点可将1#家系和6#家系与其他家系相区别;Pot14位点和Pot17位点可将3#家系与其他家系相区别;Pot42位点可将5#与其他家系相区别;Pot17位点和Pot25可将2#家系与其他家系相区别。因此,Pot18位点、Pot14位点和Pot17位点、Pot42位点、Pot17位点和Pot25位点可分别用于鉴别1#和6#家系、3#、5#、2#家系的特异性标记。研究表明,在选用的6个微卫星标记中,最少选用3个标记可鉴别6个三疣梭子蟹家系。 相似文献
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王廷春 《南京气象学院学报》2012,4(6):569-572
对非线性Schrödinger方程给出了一个线性化紧致差分格式,运用不动点定理和能量方法证明了格式的唯一可解性,还运用能量方法和数学归纳法,避开困难的先验估计,证明格式在空间方向和时间方向分别具有四阶和二阶精度,数值算例验证了格式的精度和数值稳定性. 相似文献
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Rolf Monheim 《GeoJournal》1998,45(4):273-287
Counting pedestrians on a street and visitors entering a store is a method used to measure the attractiveness of these places.
Their volumes are governed by daily, weekly, monthly and yearly cycles and are subject to random influences. This must be
taken into account especially where comparison counts reveal changes. When single streets or stores are counted, it must be
ascertained whether this figure is representative of the whole city centre and its retailing. Pedestrian volumes can be used
for adjusting interview samples to the distribution in time and space of the city centre visitors. Counts of the qualitative
characteristics of the pedestrian with respect to composition of groups reveal that surveys among pedestrians are biased in
relation to the size of the group.
Special attention in surveys should be given to the linking of activities. Whereas much emphasis is given to the accessibility
of the city centre from outside, the ‘inner accessibility’ of the city centre is just as important. Long distances are often
walked and many destinations visited. This is best recorded by conducting interviews at public transport stops and car parks.
The attractiveness of the city centre depends not only on its physical structures but very much on the subjective perception
of them; therefore judgements on accessibility, goods and services offered and the urban ambiance should be recorded. Retailer's
attitudes concerning the qualities of the city centre should also be compiled for comparison.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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夏威夷群岛是世界上著名的岛屿旅游地,是以休闲、娱乐为主要旅游目的的地区之一.通过美国夏威夷州2003 Annual Visitor Research Report,获得第二手关于夏威夷旅游者的详细数据资料,依据旅游者行为特征的研究方法,对数据进行技术处理,找出夏威夷群岛主要客源地（Major Market Areas,MMA）游客行为特征,旨在对我国海岛旅游业发展有所启示. 相似文献
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空中三角测量数据主要是为摄影测量提供定向框架数据,其质量决定最终产品的质量。笔者结合生产实践中遇到的实际问题,对提高空中三角测量数据成果质量的3个关键环节——航外控制测量,航内控制点量测,外业控制点的残差分析、空中三角测量成果检查及评定的重要性进行了阐述。 相似文献
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《Geomechanics and Geoengineering》2013,8(4):254-273
Limit equilibrium method (LEM) is the most conventional and popular technique in slope stability analysis. Location of the unique failure surface and the determination of corresponding critical factor of safety (critical-FOS) of the soil slope requires a lot of trial and research. With this amount of trial and research results, we evaluate the field of factor of safety (FOS-field), which represents the critical-FOS at a point within the domain of all possible failure surfaces. Such a field can be very important for the precise judgment of the most critical-FOS of the slope and its perturbation. This paper also presents the evaluation of the FOS-field of vegetated slopes, thus providing an analytical way to examine the effect of vegetation on the soil slope stability. We evaluated the stability of vegetated and barren soil slopes under dry and fully saturated conditions. With dry and fully saturated conditions, the behaviour of slopes in most favourable and worst conditions can be simulated. Evaluation of FOS-field for various slope geometries and conditions show that the more unstable the slope becomes, the more difficult it will be to pinpoint the location of the critical failure surface with the unique least FOS. We verified the LEM codes with finite element method (FEM) and spectral element method (SEM) codes considering a sample problem from Smith and Griffiths’ book (Smith and Griffiths 2003). 相似文献
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Eric Neumayer 《The Professional geographer》2013,65(2):171-181
The vast majority of states impose visa restrictions on travelers from some foreign countries. Such restrictions are likely to deter foreign visitors from affected countries. They will therefore reduce the flow of tourists, businesspeople, and other travelers and thereby damage a country's tourism industry, reduce its trade, and affect its scientific, cultural, and other exchanges with foreign countries. This study estimates the damaging effect exerted by visa restrictions on bilateral travel in a country dyad data set covering the period from 1995 to 2005. It finds that, depending on the exact model specification chosen, visa restrictions reduce such travel by on average between 52 and 63 percent but with substantial regional variation. Given this large detrimental effect of visa restrictions on bilateral travel, the article discusses the determinants of why states impose such restrictions. 相似文献
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将各向异性岩体中任意形状隧洞的位移解析解与智能算法--差分进化法（DE）结合,研究横观各向同性岩体中在各向异性面上开挖马蹄形隧洞的位移反分析问题,获得关于参数灵敏度、参数可惟一辨识性和测线布置方案的有关结论。计算发现,通过DE算法可获得很高精度的参数辨识结果;参数的可惟一辨识性不仅与灵敏度有关,还与具体的参数组合形式有关,在给定竖向地应力和各向同性面角度的情况下最多可以高精度惟一辨识5个参数;当待辨识参数的个数大于等于4时会产生不可完全高精度惟一辨识的参数组合,当待辨识参数的个数小于等于3时,任意的参数组合形式均可以被高精度惟一辨识。 相似文献
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酰化高丝氨酸内酯化合物(acyl-homoserine lactone,AHL)是革兰氏阴性细菌(G-)群体感应的信号分子。本文建立1种从环境微生物中高效地筛选AHL降解菌株的方法。首先利用AHL作为唯一能源对环境微生物进行初筛,再利用顶层琼脂法和报告菌株Chromobacterium violaceumATCC 12472、Agrobacterium tumefaciensA136等进行复筛,共筛选得到21株具有AHL降解活性的菌株。对其中活性最高的菌株A11进行初步的菌种鉴定,通过形态观察、16S ribosomal RNA(16S rDNA)基因分析最终确定为假单胞菌属细菌Pseudomonassp.A11。 相似文献